32 beagle dogs were locked in metal cages for 52 weeks. They were given Sucralose mixed in with their normal feed, and blood and urine samples were collected. At the end of the study they were killed by means of exsanguiation - they had their throats slit open and bled to death. They were then cut open and their organs - by now drained of blood so easier to dissect - were examined to test the product's toxicity levels.
Four beagle puppies (or as HLS calls them – punching bags) were starved before being force-fed the super-sweet sugar powder. HLS employees then took blood samples from the jugulars of the infant dogs.
An unspecified number of marmoset monkeys either died from the poisoning or were killed at the termination of the research at HLS.
The report states that 12 of these monkeys, which were babies – under 10 months old – were force-fed Sucralose for seven weeks. Two of the primates died on the seventh day from brain defects, another primate was mysteriously killed after four weeks, and the remainder all were murdered at the completion of the seventh week. Some of the recorded observations from this study noted “in appetence, body weight loss, unwillingness to use hind leg, hopping, involuntary grip reflexes, salivation and subdued mood.”
Huntingdon also used rabbits to study the effects of the product. These little animals were given 1200 times the expected daily intake and not surprisingly most died from the trauma. Many of the other rabbits suffered from convulsions, weight loss, and various intestinal disorders.
Huntingdon also tested the product on pregnant rabbits, mice, and rats – killing both the mothers and the fetuses.